3-Ketoglucose Reductase of Agrobacterium tumefaciens
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چکیده
منابع مشابه
Ferrisiderophore reductase activity in Agrobacterium tumefaciens.
Reduction of the iron in ferriagrobactin by the cytoplasmic fraction of Agrobacterium tumefaciens strictly required NaDH as the reductant. Addition of flavin mononucleotide and anaerobic conditions were necessary for the reaction; when added with flavin mononucleotide, magnesium was stimulatory. This ferrisiderophore reductase activity may be a part of the iron assimilation process in A. tumefa...
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Zinc and copper ions at 1.0 mM effectively inhibited synthesis of 3-ketodisaccharides (3-KD) by washed cells of Agrobacterium tumefaciens. Cu2+ at 0.1 mM completely inactivated cell·free preparations of the synthetic enzyme, hexopyranoside: cytochrome c oxidoreduc· tase. This ion also inhibited uptake of disaccharides. Since Zn2+ blocked disaccharide uptake but had no effect on the synthetic en...
متن کاملConditions for production of 3-ketomaltose from Agrobacterium tumefaciens.
Up to 39% yields of 3-ketomaltose were achieved in 18 to 22 hr when Agrobacterium tumefaciens NRRL B-36 was cultured at 25 to 28 C in a simple medium containing 4.0 to 8.0% maltose, 0.09% urea, 0.5% CaCO(3), 0.6% KH(2)PO(4), and 0.025% MgSO(4).7H(2)O. For maximum production of 3-ketomaltose the culture had to be maintained approximately at pH 7.0.
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Agrobacterium tumefaciens biovar 3 causes both crown gall and root decay of grapes. All biovar 3 strains, regardless of their tumorigenicity, produce in culture a single polygalacturonase with a pI around 4.5. A. tumefaciens biovar 3 strain CG49 was mutagenized with Tn5 by using pSUP2021 as a suicide vector. A mutant strain, CG50, lacking polygalacturonase activity was isolated. The mutation wa...
متن کاملMurine toxicit of Agrobacterium tumefaciens.
Eleven strains of the crown gall organism, Agrobacterium tumefaciens, tested by intraperitoneal injection into mice, were lethal within 48 hr. Five other species had some lethal strains. The lethal effect of A. tumefaciens appeared to be the result of a toxic rather than an infectious process, since histopathological anomalies were not found in mice injected with live cultures and since heat-ki...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1973
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.113.2.652-657.1973